S2-Biological Laboratory

Basic equipment for biological laboratory

• Laminar box Herasafe 2030i
• CO₂-incubator (hypo- and hyperoxic), bacteriological incubators
• Multifuge X1R Pro refrig
• Autoclave

Microscopy

• Cell culture microscope Axiovert (Zeiss)
• Fluorescence microscope Axiotech (Zeiss)
• Laser scanning microscope LSM 700 (Zeiss)
• Rotary microtome RM2265 (Leica)

Measurement technology

• Microplate-multimode reader Infinite M1000 PRO with Alpha Screen-module (Tecan)
• Multimode-reader Genios PRO (Tecan)
• Amersham ImageQuant 800 for histographic evaluation (Cytiva)
• Image analyzer software (ImagePro, Topography-software ConfoMap)

Protein analytics

• Protein electrophoresis system (Bio-Rad)
• Western-blotting system (semidry, Tankblot)
• Multi Flo FX Multimode Dispenser (Agilent)

Nucleic acid analytics

• Submersed nucleic acid gel electrophoretic system
• Endpoint PCR cycler with gradient PCR (Eppendorf)
• gPCR RotorGene (Qiagen)
• digital PCR Qiacuity One

Applications

• Quantitative ELISA-Assays
• Determination of enzyme activity
• DNA-detection
• Gene expression analyses
• ATP-luminescence-assays